Datasheet

MICA Antibody
CATALOG NUMBER: 4261

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
 2) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 42 kDa

Observed: 46 kDa
IMMUNOGEN:MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA.

The immunogen is located within the last 50 amino acids of MICA.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MICA Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MICA Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MICA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MICA Antibody: PERB11.1, PERB11.1, MHC class I polypeptide-related sequence A, MIC-A
ACCESSION NO.:NP_000238
PROTEIN GI NO.:4557751
OFFICIAL SYMBOL:MICA
GENE ID:4276

Background

BACKGROUND:MICA Antibody: Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
REFERENCES: 1) Rudolph MG, Stanfield RL and Wilson IA. How TCRs bind MHCs, peptides, and coreceptors. Annu. Rev. Immunol. 2006; 24:419-66.
2) Bahram S, Bresnahan M, Geraghty DE, et al. A second lineage of mammalian major histocompatibility complex I genes. Proc. Natl. Acad. Sci. USA 1994; 91:6259-63.
3) Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science 1999; 285:727-9.
4) Maccalli C, Pende D, Castelli C, et al. NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity. Eur. J. Immunol. 2003; 33:2033-43.

For Research Use Only