Datasheet

MettL7B Antibody
CATALOG NUMBER: 4891

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:MettL7b antibody can be used for detection of mettl7b by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1320 – Human Colon Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 27 kDa

Observed: 27 kDa
SPECIFICITY:This MettL7B antibody is predicted to not cross-react with MettL7A.
IMMUNOGEN:MettL7B antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human MettL7B.

The immunogen is located within amino acids 20 - 70 of MettL7B.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MettL7B Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MettL7B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MettL7B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MettL7B Antibody: ALDI, Methyltransferase-like protein 7B
ACCESSION NO.:NP_689850
PROTEIN GI NO.:164663805
OFFICIAL SYMBOL:METTL7B
GENE ID:196410

Background

BACKGROUND:MettL7B Antibody: MettL7B belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.
REFERENCES: 1) Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res.2003; 13:2265-70.
2) Li E, Bestor TH, and Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell1992; 69:915-26.
3) Laird PW and Jaenisch R. DNA Methylation and Cancer. Human Molecular Genetics1994; 3:1487-95.

For Research Use Only