Datasheet

MEK1/2 Antibody
CATALOG NUMBER: 8207

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Chicken: (87%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 43 kDa

Observed: 44 kDa
SPECIFICITY:MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2.
IMMUNOGEN:MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1.

The immunogen is located within amino acids 240 - 290 of MEK1/2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MEK1/2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MEK1/2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Mitogen-activated protein kinase kinase 1, MAP2K1, MAPKK1, MEK1, MKK1, PRKMK1, CFC3, Mitogen-activated protein kinase kinase 2, MAP2K2, MAPKK2, MEK2, MKK2, PRKMK2, CFC
ACCESSION NO.:NP_002746
PROTEIN GI NO.:5579478
OFFICIAL SYMBOL:MAP2K1
GENE ID:5604

Background

BACKGROUND:The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals (2). MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (2).
REFERENCES: 1) Zheng CF and Guan KL. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 1993; 268:11435-9.
2) Marais R and Marshall CJ. Control of the ERK MAP kinase cascade by Ras and Raf. Cancer Surv. 1996; 27:101-25.

For Research Use Only