Datasheet

MEIG1 Antibody
CATALOG NUMBER: 6381

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:MEIG1 antibody can be used for detection of MEIG1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1402 - Mouse Lung Tissue Lysate
IMMUNOGEN:MEIG1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminal of human MEIG1.

The immunogen is located within the last 50 amino acids of MEIG1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MEIG1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MEIG1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MEIG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MEIG1 Antibody: SPATA39, bA2K17.3, Meiosis expressed gene 1 protein homolog
ACCESSION NO.:Q5JSS6
PROTEIN GI NO.:124249370
OFFICIAL SYMBOL:MEIG1
GENE ID:644890

Background

BACKGROUND:MEIG1 Antibody: MEIG1, a murine gene first identified as a testis specific gene, is a chromosome/chromatin-binding protein initially expressed during meiosis but retained in the germ cell nucleus throughout later stages of spermatogenesis. MEIG1 is a highly conserved basal metazoan gene that is indispensable for mouse spermatogenesis. It is important for normal meiotic differentiation and absolutely crucial for terminal differentiation of spermatozoa. MEIG1 encodes two alternative transcripts, designated 2a2 and 11a2, both of which encode for a common ORF but differing in their 5' untranslated region (5'UTR) due to alternative promoters.
REFERENCES: 1) Don J and Wolgemuth DJ. Identification and characterization of the regulated pattern of expression of a novel mouse gene, meg1, during the meiotic cell cycle. Cell Growth Differ. 1992; 3:495-505
2) Salzberg Y, Eldar T, Karminsky OD, et al. Meig1 deficiency causes a severe defect in mouse spermatogenesis. Dev. Biol. 2010; 338:158-67.
3) Zhang Z, Shen X, Gude DR, et al. MEIG1 is essential for spermiogenesis in mice. Proc. Natl. Acad. Sci. USA 2009; 106:17055-60.
4) Ever L, Steiner R, Shalom S, et al. Two alternatively spliced Meig1 messenger RNA species are differentially expressed in the somatic and in the germ-cell compartments of the testis. Cell Growth Differ. 1999; 10:19-26.

For Research Use Only