Datasheet

MED4 Antibody
CATALOG NUMBER: 4729

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (86%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:MED4 antibody can be used for detection of MED4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
SPECIFICITY:At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
IMMUNOGEN:MED4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4.

The immunogen is located within the last 50 amino acids of MED4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MED4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MED4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MED4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MED4 Antibody: ARC36, VDRIP, DRIP36, TRAP36, HSPC126, ARC36, Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component
ACCESSION NO.:NP_054885
PROTEIN GI NO.:7661788
OFFICIAL SYMBOL:MED4
GENE ID:29079

Background

BACKGROUND:MED4 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.
REFERENCES: 1) Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.
2) Gustafsson CM, Myers LC, Beve J, et al. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem.1998; 273:30851-4.
3) Rachez C, Lemon BD, Suldan Z, et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature1999; 398:824-8.
4) Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

For Research Use Only