MECR Antibody Cat. No.: 7151

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: Rabbit polyclonal MECR antibody was raised against a 15 amino acid peptide near the carboxy terminus of human MECR.

The immunogen is located within amino acids 300 - 350 of MECR.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: MECR antibody can be used for detection of MECR by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of MECR are known to exist; this antibody will detect both isoforms.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 1403 - Mouse Brain Tissue Lysate
 3) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 41 kDa

psi-iconProperties
PURIFICATION:MECR Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MECR Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MECR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:MECR
ALTERNATE NAMES:MECR Antibody: NRBF1, CGI-63, FASN2B, NBRF1, Trans-2-enoyl-CoA reductase, mitochondrial, Nuclear receptor-binding factor 1, HsNrbf-1
ACCESSION NO.:NP_057095
PROTEIN GI NO.:67078404
GENE ID:51102
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.
REFERENCES:1) Masuda N, Yasumo H, Furusawa T, et al. Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors. Gene 1998; 221:225-33.
2) Chen Z, Leskinen H, Liimatta E, et al. Myocardial overexpression of Mecr, a gene of mitochondrial FAS II leads to cardiac dysfunction in mouse. PLoS One 2009; 4:e5589.

ANTIBODIES FOR RESEARCH USE ONLY.

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