Datasheet

MECR Antibody
CATALOG NUMBER: 7151

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:MECR antibody can be used for detection of MECR by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 1403 - Mouse Brain Tissue Lysate
 3) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 41 kDa
SPECIFICITY:At least two isoforms of MECR are known to exist; this antibody will detect both isoforms.
IMMUNOGEN:Rabbit polyclonal MECR antibody was raised against a 15 amino acid peptide near the carboxy terminus of human MECR.

The immunogen is located within amino acids 300 - 350 of MECR.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MECR Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MECR Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MECR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MECR Antibody: NRBF1, CGI-63, FASN2B, NBRF1, Trans-2-enoyl-CoA reductase, mitochondrial, Nuclear receptor-binding factor 1, HsNrbf-1
ACCESSION NO.:NP_057095
PROTEIN GI NO.:67078404
OFFICIAL SYMBOL:MECR
GENE ID:51102

Background

BACKGROUND:MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.
REFERENCES: 1) Masuda N, Yasumo H, Furusawa T, et al. Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors. Gene 1998; 221:225-33.
2) Chen Z, Leskinen H, Liimatta E, et al. Myocardial overexpression of Mecr, a gene of mitochondrial FAS II leads to cardiac dysfunction in mouse. PLoS One 2009; 4:e5589.

For Research Use Only