Datasheet

ME2 Antibody
CATALOG NUMBER: 7723

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 64 kDa

Observed: 60 kDa
SPECIFICITY:ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.
IMMUNOGEN:ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.

The immunogen is located within amino acids 340 - 390 of ME2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ME2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ME2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ME2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ME2 Antibody: ODS1, NAD-dependent malic enzyme, mitochondrial, Malic enzyme 2, NAD-ME
ACCESSION NO.:NP_002387
PROTEIN GI NO.:4505145
OFFICIAL SYMBOL:ME2
GENE ID:4200

Background

BACKGROUND:ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).
REFERENCES: 1) Loeber G, Infante AA, Maurer-Fogy I, et al. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J. Biol. Chem. 1991; 266:3016-21.
2) Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
3) Jiang P, Du W, Mancuso A, et al. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 2013; 493:689-83.
4) Lenzen KP, Heils A, Lorenz S, et al. Association analysis of malic acid enzyme 2 gene polymorphisms with idiopathic generalized epilepsy. Epilepsia 2005; 46:1637-41.

For Research Use Only