Datasheet

ME1 Antibody
CATALOG NUMBER: 7721

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 63 kDa

Observed: 63 kDa
SPECIFICITY:ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.
IMMUNOGEN:ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1.

The immunogen is located within the first 50 amino acids of ME1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ME1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ME1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ME1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ME1 Antibody: MES, HUMNDME, NADP-dependent malic enzyme, Malic enzyme 1, NADP-ME
ACCESSION NO.:NP_002386
PROTEIN GI NO.:4505143
OFFICIAL SYMBOL:ME1
GENE ID:4199

Background

BACKGROUND:ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).
REFERENCES: 1) Loeber G, Dworkin MB, Infante A, et al. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994; 344:181-6.
2) Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
3) Gonzalez-Manchon C, Butta N, Ferrer M, et al. Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness. DNA Cell Biol. 1997;16:533-44.
4) Yang Z, Lanks CW and Tong L. Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate. Structure 2002; 10:951-60.

For Research Use Only