MD-2 Antibody Cat. No.: 3289

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-MD-2 antibody (3289) was raised against a peptide corresponding to 13 amino acids near the center of human MD-2.

The immunogen is located within the last 50 amino acids of MD-2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-1 μg/mL; IHC: 2-2.5 μg/mL; IF: 10-20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 2) Cat. No. 1466 - Rat Spleen Tissue Lysate
 3) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 19kD

Observed: 26 kD (Post-modification: 2 N-linked glycosylations)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 1): Anti-MD-2 antibodies (3289) detected human MD-2 recombinant protein at different concentrations.

ISOFORMS: Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). 3289 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:MD-2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MD-2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MD-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LY96
ALTERNATE NAMES:MD-2 Antibody: MD2, MD-2, ly-96, ESOP-1, ESOP1, MD2, Lymphocyte antigen 96, Ly-96
ACCESSION NO.:NP_056179
PROTEIN GI NO.:223555998
GENE ID:23643
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:MD-2 Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
REFERENCES:1) O’Neill et al. Trends in Imm. 2003; 24:286-9.
2) Vogel. Mol. Interv. 2003; 3:466-77.
3) Takeda et al. Annu. Rev. Immunol. 2003; 21:335-76.
4) Janeway et al. Annu. Rev. Immunol. 2002; 20:197-216.

ANTIBODIES FOR RESEARCH USE ONLY.

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