MD-2 Antibody [1A2E3] Cat. No.: PM-4847

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
TESTED APPLICATIONS: ELISA, ICC, IF, IHC-P, WB
APPLICATIONS: MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in recombinnat protein; Immunohistochemistry in human samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 95-108 - MD-2 Recombinant Protein
 2) Cat. No. 10-901 - Human Spleen Tissue Slide

psi-iconProperties
PURIFICATION:MD-2 Monoclonal Antibody is Protein A purified.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MD-2 monoclonal antibody can be stored at -20˚C, stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:LY96
ALTERNATE NAMES:MD-2 Antibody [1A2E3] : MD2, MD-2, ly-96, ESOP-1
ACCESSION NO.:NP_056179
PROTEIN GI NO.:223555998
GENE ID:23643
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
REFERENCES:1) O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
2) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
3) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
4) Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.

ANTIBODIES FOR RESEARCH USE ONLY.

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