Datasheet

MCG10 Antibody
CATALOG NUMBER: 2479

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
IMMUNOGEN:MCG10 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10.

The immunogen is located within the last 50 amino acids of MCG10.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MCG10 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MCG10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:MCG10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MCG10 Antibody: CBP, LIP4, MCG10Alpha-CP4
ACCESSION NO.:EAW65166
PROTEIN GI NO.:119585570
OFFICIAL SYMBOL:PCBP4
GENE ID:57060

Background

BACKGROUND:MCG10 Antibody: MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.
REFERENCES: 1) Makeyev AV and Liebhaber SA. The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA2002; 8:265-78.
2) Zhu J and Chen X. MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is capable of inducing apoptosis and cell cycle arrest in G2-M. Mol. Cell Biol.2000; 20:5602-5618.
3) Pio R, Zudaire I, Pino I, et al. a-CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant aCP-4a, is underexpressed in lung cancer. Cancer Res.2004; 64:4171-9.

For Research Use Only