MBD3 Antibody [106B691] Cat. No.: 49-304

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Gorilla, Horse, Human, Orangutan, Pig, Xenopus
IMMUNOGEN: MBD3 monoclonal antibody was raised against amino acids 215 - 230 of MBD3 (Human).
TESTED APPLICATIONS: IHC, WB
APPLICATIONS: MBD3 antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 μg/mL.
SPECIFICITY: The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).

psi-iconProperties
PURIFICATION:Protein G Column
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS, 0.02% sodium azide.
CONCENTRATION:0.5 mg/ml
STORAGE CONDITIONS:MBD3 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:MBD3
ALTERNATE NAMES:MBD3
ACCESSION NO.:O95983
PROTEIN GI NO.:50400820
GENE ID:53615
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

ANTIBODIES FOR RESEARCH USE ONLY.

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