Datasheet

MAPKAP1 Antibody
CATALOG NUMBER: 4079

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:MAPKAP1 antibody can be used for detection of MAPKAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
IMMUNOGEN:MAPKAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human MAPKAP1.

The immunogen is located within amino acids 340 - 390 of MAPKAP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MAPKAP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MAPKAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MAPKAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MAPKAP1 Antibody: MIP1, SIN1, JC310, SIN1b, SIN1g, MIP1, Target of rapamycin complex 2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, TORC2 subunit MAPKAP1
ACCESSION NO.:NP_001006618
PROTEIN GI NO.:56788407
OFFICIAL SYMBOL:MAPKAP1
GENE ID:79109

Background

BACKGROUND:MAPKAP1 Antibody: MAPKAP1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
REFERENCES: 1) Schroder W, Cloonan N, Bushell G, et al. Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene. Gene2004; 339:17-23.
2) Cheng J, Zhang D, Kim K, et al. Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation. Mol. Cell. Biol.2005; 25:5955-64.
3) Jacinto E, Facchinetti V, Liu D, et al. SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell2006; 127:125-37.
4) Wullschleger S, Loewith R and Hall MN. TOR signaling in growth and metabolism. Cell2006; 124:471-84.

For Research Use Only