Datasheet

MAP1LC3C Antibody
CATALOG NUMBER: 7583

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MAP1LC3C antibody can be used for detection of MAP1LC3C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 16 kDa

Observed: 16 kDa
SPECIFICITY:MAP1LC3C antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3C are known to exist. MAP1LC3C antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3B.
IMMUNOGEN:MAP1LC3C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human MAP1LC3C.

The immunogen is located within the last 50 amino acids of MAP1LC3C.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MAP1LC3C antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MAP1LC3C antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MAP1LC3C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MAP1LC3C Antibody: LC3C, ATG8JAutophagy-related protein LC3 C
ACCESSION NO.:NP_001004343
PROTEIN GI NO.:51972260
OFFICIAL SYMBOL:MAP1LC3C
GENE ID:440738

Background

BACKGROUND:Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3C belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3C is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3C, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).
REFERENCES: 1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
2) Halpain S and Dehmelt L. The MAP1 family of microtubule-associated proteins. Genome Biol. 2006; 7:224.
3) He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.

For Research Use Only