MAP1LC3 Antibody Cat. No.: 7951

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: MAP1LC3 antibody was raised against a 12 amino acid peptide near the center of human MAP1LC3A.

The immunogen is located within amino acids 20 - 70 of MAP1LC3.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: MAP1LC3 antibody can be used for detection of MAP1LC3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
SPECIFICITY: MAP1LC3 antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3 are known to exist. MAP1LC3 antibody is predicted to detect MAP1LC3A, MAP1LC3B, and MAP1LC3C.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 13 kDa

Observed: 18 kDa

psi-iconProperties
PURIFICATION:MAP1LC3 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:MAP1LC3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MAP1LC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:MAP1LC3A
ALTERNATE NAMES:MAP1LC3 Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein LC3 A
ACCESSION NO.:NP_115903
PROTEIN GI NO.:14210522
GENE ID:84557
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3 is a subfamily of three related proteins belonging to the MAP1 LC3 family and it includes MAP1LC3A, MAP1LC3B, and MAP1LC3C (2). MAP1LC3 is the mammalian homolog of yeast ATG8 and is essential for autophagy and associated with the autophagosome membranes after processing (3). The three isoforms exhibit distinct expression patterns and both MAP1LC3A and MAP1LC3B but not MAP1LC3B, are post-translationally modified, suggesting the three isoforms may have different physiological functions (4).
REFERENCES:1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
2) Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85.
3) Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8.
4) He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.