Datasheet

MAK10 Antibody
CATALOG NUMBER: 5273

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:MAK10 antibody can be used for detection of MAK10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
IMMUNOGEN:MAK10 antibody was raised against a 14 amino acid synthetic peptide near the center of human MAK10.

The immunogen is located within amino acids 420 - 470 of MAK10.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MAK10 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MAK10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MAK10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MAK10 Antibody: EGAP, MAK10, MAK10P, bA379P1.1, EGAP, N-alpha-acetyltransferase 35, NatC auxiliary subunit, Embryonic growth-associated protein homolog
ACCESSION NO.:NP_078911
PROTEIN GI NO.:145275204
OFFICIAL SYMBOL:MAK10
GENE ID:60560

Background

BACKGROUND:MAK10 Antibody: The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.
REFERENCES: 1) Lee YJ et al. MAK10, a glucose repressible gene necessary for replication of a dsRNA virus of Saccharomyces cerevisiae, has T cell receptor alpha-subunit motifs. Genetics1992; 132:87-96.
2) Yi XJ, et al. A novel epithelial wound-related gene is abundantly expressed in developing rat cornea and skin. Curr. Eye Res.2000; 20:430-40.
3) Wenzlau JM, et al. Embryonic growth-associated protein is one subunit of a novel N-terminal acetyltransferase complex essential for embryonic vascular development. Circ. Res.2006; 98:846-55.
4) Weiser-Evans MC, et al. Novel embryonic genes are preferentially expressed by autonomously replicating rat embryonic and neointimal smooth muscle cells. Circ. Res.2000; 87:608-15.

For Research Use Only