Datasheet

Maelstrom Antibody
CATALOG NUMBER: 5667

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:Maelstrom antibody can be used for detection of Maelstrom by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
IMMUNOGEN:Maelstrom antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Maelstrom.

The immunogen is located within amino acids 30 - 80 of Maelstrom.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Maelstrom Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Maelstrom Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Maelstrom antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Maelstrom Antibody: CT128, SPATA35, Protein maelstrom homolog
ACCESSION NO.:AAY82463
PROTEIN GI NO.:67866977
OFFICIAL SYMBOL:MAEL
GENE ID:84944

Background

BACKGROUND:Maelstrom Antibody: The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.
REFERENCES: 1) Clegg NJ, Frost DM, Larkin MK, et al. Maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA. Development1997; 124:4661-71.
2) Costa Y, Speed RM, Gautier P, et al. Mouse MAELSTROM: the link between meiotic silencing of unsynapsed chromatin and microRNA pathway? Hum. Mol. Genet.2006; 15:2324-34.
3) Soper SFC, van der Heijen GW, Hardiman TC, et al. Mouse Maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev. Cell2008; 15:285-97.
4) Xiao L, Wang Y, Zhou Y, et al. Identification of a novel human/testis gene MAEL that is regulated by DNA methylation. Mol. Biol. Rep.2009; epub.

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