Datasheet

MACC1 Antibody
CATALOG NUMBER: 5197

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MACC1 antibody can be used for detection of MACC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
 3) Cat. No. 1304 – Human Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 94 kDa

Observed: 90 kDa
SPECIFICITY:At least two isoforms of MACC1 are known to exist.
IMMUNOGEN:MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1.

The immunogen is located within amino acids 430 - 480 of MACC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MACC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MACC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MACC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MACC1 Antibody: 7A5, SH3BP4L, Metastasis-associated in colon cancer protein 1, SH3 domain-containing protein 7a5
ACCESSION NO.:NP_877439
PROTEIN GI NO.:157502191
OFFICIAL SYMBOL:MACC1
GENE ID:346389

Background

BACKGROUND:MACC1 Antibody: Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment.
REFERENCES: 1) Stein U, Walther W, Arlt F, et al. MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis. Nat. Med.2009; 15:59-67.
2) Boardman LA. Overexpression of MACC1 leads to downstream activation of HGF/MET and potentiates metastasis and recurrence of colorectal cancer. Genome Med.2009; 1:36.
3) Stein U, Smith J, Walther W, et al. MACC1 controls Met: what a difference an Sp1 site makes. Cell Cycle2009; 8:2467-9.
4) Arlt F and Stein U. Colon cancer metastasis: MACC1 and Met as metastatic pacemakers. Int. J. Biochem. Cell Biol.2009; epub.

For Research Use Only