Datasheet

LXR-A Antibody
CATALOG NUMBER: 5577

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:LXR-A antibody can be used for detection of LXR-A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 44, 49 kDa

Observed: 46 kDa
IMMUNOGEN:LXR-A antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus human LXR-A.

The immunogen is located within amino acids 50 - 100 of LXR-A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LXR-A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LXR-A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LXR-A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LXR-A Antibody: LXRA, LXR-a, RLD-1, LXRA, Oxysterols receptor LXR-alpha, Liver X receptor alpha
ACCESSION NO.:NP_005684
PROTEIN GI NO.:194294517
OFFICIAL SYMBOL:NR1H3
GENE ID:10062

Background

BACKGROUND:LXR-A Antibody: LXR-A belongs to the Liver X Receptor family that encodes highly homologous transcription factors. Like the highly homologous LXR-B, LXR-A forms heterodimers with the retinoic acid receptor RXRalpha, function as sensors for cellular oxysterols which when activated, increase the expression of genes that control sterol and fatty acid metabolism and homeostasis. Recent experiments have indicated that the LXRs can also modulate both innate and adaptive immune responses. Human and mouse tumors produce LXR ligands that inhibit CCR7 expression on maturing dendritic cells (DCs), thereby allowing tumor immunoescape. In mouse models, it was shown that ablating LXR-A signaling led to an immune-mediated strong inhibition of tumor growth, suggesting that manipulation of this pathway may be a viable anti-cancer approach.
REFERENCES: 1) Willy PJ, Umesono K, Ong ES, et al. LXR, a nuclear receptor that defines a distinct retinoid response pathway. Genes Dev.1995; 9:1033-45.
2) Edwards PA, Kennedy MA, and Mak PA. LXRs; Oxysterol-activated nuclear receptors that regulate genes controlling lipid homeostasis. Vasc. Pharmacol.2002; 38:249-56.
3) Bensinger SJ and Tontonoz P. Integration of metabolism and inflammation by lipid-activated nuclear receptors. Nature2008; 454:470-7.
4) Villablanca EJ, Raccosta L, Zhou D, et al. Tumor-mediated liver X receptor-a activation inhibits CC chemokine reeptor-7 expression on dendritic cells and dampens antitumor responses. Nature Med.2010; 16:98-106.

For Research Use Only