LRRFIP2 Antibody Cat. No.: 6221

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: LRRFIP2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LRRFIP2.

The immunogen is located within amino acids 620 - 670 of LRRFIP2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: LRRFIP2 antibody can be used for detection of LRRFIP2 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Three isoforms of LRRFIP2 are known to exist; this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members.
POSITIVE CONTROL:1) Cat. No. 1472 - Rat Colon Tissue Lysate

psi-iconProperties
PURIFICATION:LRRFIP2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LRRFIP2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LRRFIP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LRRFIP2
ALTERNATE NAMES:LRRFIP2 Antibody: HUFI-2, Leucine-rich repeat flightless-interacting protein 2, LRR FLII-interacting protein 2
ACCESSION NO.:NP_006300
PROTEIN GI NO.:5453726
GENE ID:9209
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:LRRFIP2 Antibody: The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of beta-catenin. LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-κB during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.
REFERENCES:1) Fong KS and de Couet HG. Novel proteins interacting with the leucine-rich repeat domain of human flightless-I identified by the yeast two-hybrid system. Genomics 1999; 58:146-57.
2) Liu J, Bang AG, Kintner C, et al. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis. Proc. Natl. Acad. Sci. USA 2005; 102:1927-32.
3) Dai P, Jeong SY, Yu Y, et al. Modulation of TLR signaling by multiple MyD88-interacting including leucine-rich repeat Fli-I-interacting proteins. J. Immunol. 2009; 182:3450-60.
4) Gunawardena HP, Huang Y, Kenjale R, et al. Unambiguous characterization of site-specific phosphorylation of Leucine-rich FLI-I-interacting protein 2 (LRRFIP2) in Toll-like receptor 4 (TLR4)-mediated signaling. J. Biol. Chem. 2011; epub.

ANTIBODIES FOR RESEARCH USE ONLY.

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