Datasheet

LRRC8A Antibody
CATALOG NUMBER: 8235

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 89 kDa

Observed: 95 kDa
SPECIFICITY:LRRC8A antibody is human, mouse and rat reactive.
IMMUNOGEN:LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A.

The immunogen is located within amino acids 60 - 110 of LRRC8A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LRRC8A antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LRRC8A antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LRRC8A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5
ACCESSION NO.:NP_062540
PROTEIN GI NO.:62241040
OFFICIAL SYMBOL:LRRC8A
GENE ID:56262

Background

BACKGROUND:The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).
REFERENCES: 1) Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13.
2) Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52.
3) Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.

For Research Use Only