LNPEP Antibody Cat. No.: 7055

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (73%), Rat: (73%)
IMMUNOGEN: LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP.

The immunogen is located within amino acids 900 - 950 of LNPEP.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT: 113 kDa

psi-iconProperties
PURIFICATION:LNPEP Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LNPEP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LNPEP
ALTERNATE NAMES:LNPEP Antibody: CAP, IRAP, PLAP, P-LAP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Cystinyl aminopeptidase
ACCESSION NO.:NP_005566
PROTEIN GI NO.:61742777
GENE ID:4012
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:LNPEP Antibody: LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
REFERENCES:1) Keller SR, Scott HM, Mastick CC, et al. Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles. J. Biol. Chem. 1995; 270:23612-8.
2) Wallis MG, Lankford MF, and Keller SR. Vasopressin is a physiological substrate for the insulin-regulated aminopeptidase IRAP. Am. J. Physiol. Endocrinol. Metab. 2007; 293:E1092-102.
3) Saveanu L, Carroll O, Weimershaus M, et al. IRAP identifies an endosomal compartment required for MHC class I cross-presentation. Science 2009; 325:213-7.
4) Jordens I, Molle D, Xiong W, et al. Insulin-regulated aminopeptidase is a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to specialized insulin-regulated vesicles. Mol. Biol. Cell 2010; 21:2034-44.

ANTIBODIES FOR RESEARCH USE ONLY.

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