LKB1 Antibody Cat. No.: 3987

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (87%)
IMMUNOGEN: LKB1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human LKB1.

The immunogen is located within amino acids 310 - 360 of LKB1.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: LKB1 antibody can be used for detection of LKB1 by Western blot at 1 μg/mL. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1216 - PC-3 Cell Lysate

psi-iconProperties
PURIFICATION:LKB1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LKB1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LKB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:STK11
ALTERNATE NAMES:LKB1 Antibody: PJS, LKB1, hLKB1, PJS, Liver kinase B1
ACCESSION NO.:AAH19334
PROTEIN GI NO.:17939640
GENE ID:6794
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:LKB1 Antibody: The LKB1 serine/threonine protein kinase was initially identified as a tumor suppressor gene mutated in human Peutz-Jeghers syndrome (PJS), a condition resulting in the growth of numerous intestinal polyps classed as hamartomas. LKB1 exists as a heterotrimeric complex with two other proteins, Ste20-related adaptor protein (STRAD) and MO25. Together, this complex can phsophorylate and activate the AMP-activate protein kinase (AMPK). Following AMPK activation by LKB1, AMPK then phosphorylates TSC1 and TSC2, key components of the metabolism-regulating TOR signaling pathway, which antagonizes the activation for the TOR pathway. LKB1 has also been shown to play a fundamental role in controlling the spatial orientation of structures required to maintain an ordered, polarized epithelium.
REFERENCES:1) Hemminki A, Markie D, Tomlinson I, et al. A serine/threonine kinase gene defective in Peutz-Jeghers syndrome. Nature 1998; 391:184-7.
2) Boudeau J, Baas AF, Deak M, et al. MO25α/β interact with STRADα/β enhancing their ability to bind, activate and localize LKB1 in the cytoplasm. EMBO J. 2003; 22:5102-14.
3) Hawley SA, Boudeau J, Reid JL, et al. Complexes between the LKB1 tumor suppressor, STRADα/β and MO25α/β are upstream kinases in the AMP-activated protein kinase cascade. J. Biol. 2003; 2:28

ANTIBODIES FOR RESEARCH USE ONLY.

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