Listeria sp. p60 Antibody [p6017] Cat. No.: 36-022

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Listeria monocytogenes, Listeria sp.
IMMUNOGEN: Recombinant Listeria spp. p60.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA: (direct or indirect: 1:5,000-1:10,000). Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
SPECIFICITY: Recognizes L. monocytogenes p60 protein. Weakly cross-reacts with other Listeria species (L. innocua, L. ivanovii).

psi-iconProperties
CLONALITY:Monoclonal
ISOTYPE:IgG1, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -20˚C.

psi-iconAdditional Info
OFFICIAL SYMBOL:iap
ALTERNATE NAMES:IAP p60 from Listeria Monocytogenes; Invasion-associated Protein p60 from Listeria Monocytogenes
ACCESSION NO.:P21171
PROTEIN GI NO.:1171970
GENE ID:985140
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.

ANTIBODIES FOR RESEARCH USE ONLY.

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