Datasheet

LIN54 Antibody
CATALOG NUMBER: 7933

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 88 kDa

Observed: 82 kDa
SPECIFICITY:LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.
IMMUNOGEN:LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.

The immunogen is located within the last 50 amino acids of LIN54.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LIN54 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LIN54 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LIN54 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LIN-54, JC8.6, CXCDC1, MIP120
ACCESSION NO.:NP_919258
PROTEIN GI NO.:169234719
OFFICIAL SYMBOL:LIN54
GENE ID:132660

Background

BACKGROUND:LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).
REFERENCES: 1) Schmit F, Cremer S, and Gaubatz S. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promotoer in a sequence-specific manner. FEBS J. 2009; 276:5703-16.
2) Matsuo T, Kuramoto H, Kumazaki T, et al. LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression. Cell Cycle 2012; 11:3227-36.

For Research Use Only