Lin28 Antibody Cat. No.: 5605

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (87%), Chicken: (79%)
IMMUNOGEN: Lin28 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Lin28.

The immunogen is located within the last 50 amino acids of Lin28.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Lin28 antibody can be used for detection of Lin28 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide

psi-iconProperties
PURIFICATION:Lin28 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Lin28 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Lin28 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LIN28A
ALTERNATE NAMES:Lin28 Antibody: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, Lin-28A
ACCESSION NO.:EAX07814
PROTEIN GI NO.:119628219
GENE ID:79727
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Lin28 Antibody: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
REFERENCES:1) Ambros V. A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell1989; 57:49-57.
2) Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
3) Tyu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science2007; 318:1917-20
4) Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.

ANTIBODIES FOR RESEARCH USE ONLY.

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