Datasheet

LIMA1 Antibody
CATALOG NUMBER: 8053

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (89%), Pig: (72%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 50, 66, 84 kDa

Observed: 65 kDa
SPECIFICITY:LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
IMMUNOGEN:LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1.

The immunogen is located within amino acids 230 - 280 of LIMA1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LIMA1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LIMA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3
ACCESSION NO.:NP_001107018
PROTEIN GI NO.:165905589
OFFICIAL SYMBOL:LIMA1
GENE ID:51474

Background

BACKGROUND:The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
REFERENCES: 1) Maul RS and Chang DD. EPLIN, epithelial protein lost in neoplasm. Oncogene 1999; 18:7838-41.
2) Maul RS, Song Y, Amann KJ, et al. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 2003; 160:399-407.
3) Abe K and Takeichi M. EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt. Proc. Natl. Acad. Sci. USA 2008; 105:13-9.
4) Zhang S, Wang X, Iqval S, et al. Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition. J. Biol. Chem. 2013; 288:1469-79.

For Research Use Only