LIGHT Antibody (biotin) Cat. No.: XP-5215Bt

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA:

Sandwich:
To detect hLIGHT by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

Western Blot:

To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

psi-iconProperties
PURIFICATION:Anti-hLIGHT specific antibody was purified by affinity chromatography and then biotinylated.
CLONALITY:Polyclonal
CONJUGATE:Biotin
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:LIGHT antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:TNFSF14
ALTERNATE NAMES:LTg, TR2, CD258, HVEML, LIGHT, UNQ391/PRO726, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L
ACCESSION NO.:O43557
PROTEIN GI NO.:313104028
GENE ID:8740
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.

ANTIBODIES FOR RESEARCH USE ONLY.

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