LIF Antibody Cat. No.: 6245

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: Anti-LIF antibody (6245) was raised against a peptide corresponding to 16 amino acids near the center of human LIF.

The immunogen is located within amino acids 50-100 of LIF.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: WB: 1-4 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of LIF are known to exist.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 22kD

Observed: 26kD (Post-modifications: N-linked Glycosylations)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 3): Anit-LIF antibodies (6245) detected human LIF recombinant protein at different concentrations.

ISOFORMS: Human LIF has 2 isoforms, including isoform 1 (202aa, 22kD) and isoform 2 (88aa, 9.6kD). Mouse LIF has 1 isoform (203aa, 22.3kD and Rat LIF also has 1 isoform (202aa, 22.1kD). 6245 can detect human isoform1 as well as mouse and rat LIF.

psi-iconProperties
PURIFICATION:LIF Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LIF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LIF
ALTERNATE NAMES:LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF
ACCESSION NO.:NP_002300
PROTEIN GI NO.:4504991
GENE ID:3976
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
REFERENCES:1) Trouillas et al. Cytokine Netw. 2009; 20:51-62.
2) Hilton et al. Anal. Biochem. 1988; 173:359-67.
3) Schemlzer et al. Protein Expr. Purif. 1990; 1:54-62.

ANTIBODIES FOR RESEARCH USE ONLY.

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