Datasheet

LEMD3 Antibody
CATALOG NUMBER: 6603

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
SPECIFICITY:At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2
IMMUNOGEN:LEMD3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LEMD3.

The immunogen is located within the last 50 amino acids of LEMD3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LEMD3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LEMD3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LEMD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LEMD3 Antibody: MAN1, MAN1, Inner nuclear membrane protein Man1, LEM domain-containing protein 3
ACCESSION NO.:NP_055134
PROTEIN GI NO.:7706607
OFFICIAL SYMBOL:LEMD3
GENE ID:23592

Background

BACKGROUND:LEMD3 Antibody: LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.
REFERENCES: 1) Hellemans J, Preobrazhenska O, Willaert A, et al. Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. Nat. Genet. 2004; 36:1213-8.

For Research Use Only