Datasheet

LEMD2 Antibody
CATALOG NUMBER: 6891

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
SPECIFICITY:At least two isoforms of LEMD2 are known to exist; this antibody will detect only the longer isoform.
IMMUNOGEN:LEMD2 antibody was raised against a 16 amino acid synthetic peptide near the center of human LEMD2.

The immunogen is located within amino acids 220 - 270 of LEMD2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LEMD2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LEMD2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LEMD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LEMD2 Antibody: NET25, dJ482C21.1, LEM domain-containing protein 2, hLEM2
ACCESSION NO.:NP_851853
PROTEIN GI NO.:31044432
OFFICIAL SYMBOL:LEMD2
GENE ID:221496

Background

BACKGROUND:LEMD2 Antibody: The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.
REFERENCES: 1) Brachner A, Reipert S, Foisner R, et al. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J. Cell Sci. 2005; 118:5797-810
2) Huber MD, Guan T, and Gerace L. Overlapping functions of nuclear envelope proteins NET25 (Lem2) and Emerin in regulation of extracellular signal-regulated kinase signaling in myoblast differentiation. Mol. Cell. Biol. 2009; 29:5718-28.

For Research Use Only