Datasheet

LEMD1 Antibody
CATALOG NUMBER: 6599

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:LEMD1 antibody can be used for detection of LEMD1 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
SPECIFICITY:At least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3.
IMMUNOGEN:LEMD1 antibody was raised against a 19 amino acid synthetic peptide near the center of human LEMD1.

The immunogen is located within amino acids 90 - 140 of LEMD1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LEMD1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LEMD1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LEMD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LEMD1 Antibody: CT50, LEMP-1, LEM domain-containing protein 1, LEM domain protein 1, CT50
ACCESSION NO.:Q68G75
PROTEIN GI NO.:312261248
OFFICIAL SYMBOL:LEMD1
GENE ID:93273

Background

BACKGROUND:LEMD1 Antibody: LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.
REFERENCES: 1) Yuki D, Lin YM, Fujii Y, et al. Isolation of LEM domain-containing 1, a novel testis-specific gene expressed in colorectal cancers. Oncol. Rep. 2004; 12:275-80
2) Ghafouri-Fard S, Ousati Ashtiani Z, Sabah Golian B, et al. Expression of two testis-specific genes, SPATA19 and LEMD1, in prostate cancer. Arch. Med. Res. 2010; 41:195-200

For Research Use Only