LDL R Recombinant Protein Cat. No.: 96-807

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
SPECIES:Cynomolgus monkey
SOURCE SPECIES:HEK293 cells
SEQUENCE:Ala 22 - Gly 788
FUSION TAG:His Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus LDL R, His Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human PCSK9, His Tag with a linear range of 0.007-0.256 ug/mL (QC tested).
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:86.8 kDa
PHYSICAL STATE:Lyophilized
BUFFER:PBS, pH7.3
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:LDLR,FH,FHC,LDLCQ2
ACCESSION NO.:XP_005588053.1
OFFICIAL SYMBOL:LDL R
GENE ID:102127361
psi-iconBackground and References
BACKGROUND:Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
REFERENCES:1) Yamamoto T, et al., 1984, Cell 39 (1): 27–38.
2) Nykjaer A, Willnow TE, 2002, Trends Cell Biol. 12 (6): 273–80.
3) Barrett P.H., Watts G.F. 2002, Atherosclerosis. Supplements. 2: 1-4.
4) Nomura S. et al., 2004, Gene. Therapy. 11: 1540-1548.

FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.