Datasheet

LDL-R Antibody
CATALOG NUMBER: 5163

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (89%), Rabbit: (80%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:LDL-R antibody can be used for detection of LDL-R by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
IMMUNOGEN:LDL-R antibody was raised against an 18 amino acid synthetic peptide near the center of human LDL-R.

The immunogen is located within amino acids 490 - 540 of LDL-R.
HOST SPECIES:Chicken

Properties

PURIFICATION:LDL-R Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LDL-R Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LDL-R antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LDL-R Antibody: FH, FHC, LDLCQ2, Low-density lipoprotein receptor, LDL receptor
ACCESSION NO.:NP_000518
PROTEIN GI NO.:4504975
OFFICIAL SYMBOL:LDLR
GENE ID:3949

Background

BACKGROUND:LDL-R Antibody: The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.
REFERENCES: 1) Li Y, Cam J, and Bu G. Low-density lipoprotein receptor family: endocytosis and signal transduction. Mol. Neurobiol.2001; 23:53-67.
2) Austin MA, Hutter CM, Zimmern RL, et al. Familial hypercholesterolemia and coronary heart disease: a HuGE association review. Am. J. Epidemiol.2004; 160:421-9.
3) Helle F and Dubuisson J. Hepatitis C virus entry into host cells. Cell Mol. Life Sci.2008; 65:100-12.

For Research Use Only