Datasheet

LASS6 Antibody
CATALOG NUMBER: 4941

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:LASS6 antibody can be used for detection of LASS6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
SPECIFICITY:At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.
IMMUNOGEN:LASS6 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human LASS6.

The immunogen is located within the last 50 amino acids of LASS6.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LASS6 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LASS6 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LASS6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LASS6 Antibody: CERS5, LASS6, Ceramide synthase 6, LAG1 longevity assurance homolog 6, CerS6
ACCESSION NO.:AAI09285
PROTEIN GI NO.:80478334
OFFICIAL SYMBOL:CERS6
GENE ID:253782

Background

BACKGROUND:LASS6 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. Like the highly homologous LASS5, LASS6 is also an endoplasmic reticulum, multi-pass membrane protein. LASS6 is also involved in the synthesis of C14, C16 and C18-ceramide, but shows a preference for unsaturated fatty acids. LASS6 is broadly expressed in a wide range of tissues and microarray data suggests that it may play a role in cancer differentiation and early embryonic development.
REFERENCES: 1) Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem.2003; 278:43452-9.
2) Mizutani Y, Kihara A and Igarashi Y. Mammalian Lass6 and its related family members regulate synthesis of specific ceramides. Biochem. J.2005; 390:263-71.
3) Lahiri S and Futerman AH. LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor. J. Biol Chem.2005; 280:33735-8.
4) Weinmann A, Galle PR, and Teufel A. LASS6, an additional member of the longevity assurance gene family. Int. J. Mol. Med.2005; 16:905-10.

For Research Use Only