Lano Antibody Cat. No.: 3001

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (87%)
IMMUNOGEN: Lano antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Lano.

The immunogen is located within the last 50 amino acids of Lano.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Lano antibody can be used for detection of Lano by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1202 – A431 Cell Lysate
 2) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 59 kDa

Observed: 59 kDa

psi-iconProperties
PURIFICATION:Lano Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Lano Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Lano antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LRRC1
ALTERNATE NAMES:Lano Antibody: LANO, dJ523E19.1, LANO, Leucine-rich repeat-containing protein 1, LANO adapter protein
ACCESSION NO.:AAK72246
PROTEIN GI NO.:14701834
GENE ID:55227
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Lano Antibody: Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble. The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells. Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions. It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues.
REFERENCES:1) Saito H, Santoni M-J, Jaulin-Bastard F, et al. Lano, a novel LAP protein directly connected to MAGUK proteins in epithelial cells. J. Biol. Chem. 2001; 276:32051-5.
2) Legouis R, Jaulin-Bastard F, Schott S, et al. Basolateral targeting by leucine-rich repeat domains in epithelial cells. EMBO Rep. 2003; 4:1096-102.
3) Funke L, Dakoji S, Bredt DS. Membrane-Associated Guanylate Kinases Regulate Adhesion and Plasticity at Cell Junctions. Annu. Rev. Biochem. 2005; 74:219-45.
4) Bilder D and Perrimon N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 2000; 403:676-80.

ANTIBODIES FOR RESEARCH USE ONLY.

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