Datasheet

LAMTOR3 Antibody
CATALOG NUMBER: 7713

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:LAMTOR3 antibody can be used for detection of LAMTOR3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 14 kDa

Observed: 13 kDa
SPECIFICITY:LAMTOR3 antibody is human, mouse and rat reactive. At least two isoforms of LAMTOR3 are known to exist; this antibody will detect both isoforms. LAMTOR3 antibody is predicted to not cross-react with other LAMTOR family proteins.
IMMUNOGEN:LAMTOR3 antibody was raised against an 15 amino acid peptide near the center of human LAMTOR3.

The immunogen is located within amino acids 40 - 90 of LAMTOR3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:LAMTOR3 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:LAMTOR3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LAMTOR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LAMTOR3 Antibody: MP1, MAPBP, MAPKSP1, PRO0633, MAP2K1IP1, Ragulator3, PRO2783, Ragulator complex protein LAMTOR3, MEK-binding partner 1, Mp1
ACCESSION NO.:NP_068805
PROTEIN GI NO.:11496277
OFFICIAL SYMBOL:LAMTOR3
GENE ID:8649

Background

BACKGROUND:The late endosomal/lysosomal adaptor MAPK and MTOR activator 3 (LAMTOR3) protein belongs to the LAMTOR family of proteins, and together with LAMTOR2 and the MAPK1 and ERK kinase 1 (MEK1) localizes to late endosomes where it is required for the efficient activation of ERK signaling (1,2). This complex is involved in the regulation of late endosomal traffic and cellular proliferation (3) and plays a role in cellular host defense against Salmonella infection (4).
REFERENCES: 1) Wunderlich W, Fialka I, Teis D, et al. A novel 14-kilodalton protein interacts with the mitogen-activated protein kinase scaffold MP1 on a late endosomal/lysosomal compartment. J. Cell Biol. 2001; 152:765-76.
2) Teis D, Wunderlich W, and Huber LA. Localization of the MP1-MAPK scaffold complex to endosomes is mediated by p14 and required for signal transduction. Dev. Cell 2002; 3:803-14.
3) Teis D, Taub N, Kurzbauer R, et al. p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis. J. Cell Biol. 2006; 175:861-8.
4) Taub N, Nairz M, Hilber D, et al. The late endosomal adaptor p14 is a macrophage host-defense factor against Salmonella infection. J. Cell Sci. 2012; 125:2698-708.

For Research Use Only