LAMTOR1 Antibody Cat. No.: 7709

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: Rabbit polyclonal LAMTOR1 antibody was raised against a 17 amino acid peptide near the amino terminus of human LAMTOR1 .

The immunogen is located within the first 50 amino acids of LAMTOR1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: LAMTOR1 antibody can be used for detection of LAMTOR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: LAMTOR1 antibody is human, mouse and rat specific. LAMTOR1 antibody is predicted to not cross-react with other LAMTOR family proteins.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 18 kDa

Observed: 18 kDa

psi-iconProperties
PURIFICATION:LAMTOR1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LAMTOR1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LAMTOR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:LAMTOR1
ALTERNATE NAMES:LAMTOR1 Antibody: p18, PDRO, C11orf59, p27RF-Rho, Ragulator1, PP7157, Ragulator complex protein LAMTOR1, Lipid raft adaptor protein p18
ACCESSION NO.:NP_060377
PROTEIN GI NO.:8923579
GENE ID:55004
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:LAMTOR1 is a 161 amino acid membrane protein belonging to the LAMTOR family. It has been identified to interact with and recruit the four Rag GTPases (Rag A-D) to the surface of lysosomes (1). The mTORC1 kinase complex is a critical component in the regulation of cell growth (2). As part of the ragulator complex, LAMTOR1 recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids (3). LAMTOR1 may be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes / lysosomes (4).
REFERENCES:1) Sancak Y, Bar-Peled L, Zoncu R, et al. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell 2010; 141:290-303.
2) Kim E, Goraksha-Hicks P, Li L, et al. Regulation of TORC1 by Rag GTPases in nutrient response. Nat. Cell Biol. 2008; 10:935-45.
3) Wullschleger S, Loewith R, and Hall MN. TOR signaling in growth and metabolism. Cell 2006; 124:471-84.
4) Soma-Nagae T, Nada S, Kitagawa M, et al. The lysosomal signaling anchor p18/LAMTOR1 controls epidermal development by regulating lysosome-mediated catabolic processes. J. Cell Sci. 2013; 126:3575-84.

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