LAMP-1 Antibody Cat. No.: 3629

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: LAMP-1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human LAMP-1.

The immunogen is located within amino acids 230 - 280 of LAMP-1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
SPECIFICITY: LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 46 kDa

Observed: 95 kDa

psi-iconProperties
PURIFICATION:LAMP-1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LAMP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LAMP-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LAMP1
ALTERNATE NAMES:LAMP-1 Antibody: LAMPA, CD107a, LGP120, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, LAMP-1
ACCESSION NO.:NP_005552
PROTEIN GI NO.:112380628
GENE ID:3916
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:LAMP-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.
REFERENCES:1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4) Chen JW, Murphy TL, Willingham MC, et al. Identification of two lysosomal membrane glycoproteins. J. Cell Biol. 1985; 101:85-95.

ANTIBODIES FOR RESEARCH USE ONLY.

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