Datasheet

LAG3 Antibody [6D1]
CATALOG NUMBER: RF16087

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ICC, IF, IHC-P
APPLICATIONS:LAG-3 antibody can be used for Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1331 - Human Lymphoma Tissue Lysate
 2) Cat. No. 1306 - Human Spleen Tissue
IMMUNOGEN:LAG-3 antibody was raised against the extracellular domain of human LAG-3
HOST SPECIES:Mouse

Properties

PURIFICATION:LAG-3 Antibody is supplied as protein A purified IgG1.
PHYSICAL STATE:Liquid
BUFFER:LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LAG-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Monoclonal
ISOTYPE:IgG1,k
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
ACCESSION NO.:NP_002277
PROTEIN GI NO.:167614500
OFFICIAL SYMBOL:LAG3
GENE ID:3902

Background

BACKGROUND:The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
REFERENCES: 1) Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
2) Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
3) Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
4) Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

For Research Use Only