LAG-3 Antibody [1A8] Cat. No.: SD8843

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psi-iconSpecifications
HOST SPECIES:Llama
SPECIES REACTIVITY: Human
IMMUNOGEN: LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
TESTED APPLICATIONS: ELISA, IF, IHC-P
APPLICATIONS: LAG-3 antibody can be used for ELISA starting at 0.1 μg/mL. For immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.
PREDICTED MOLECULAR WEIGHT: Predicted: 55 kDa

psi-iconProperties
PURIFICATION:LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.
CLONALITY:Monoclonal
ISOTYPE:sdAb
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:LAG-3 Antibody is supplied in PBS.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:LAG3 antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:LAG3
ALTERNATE NAMES:LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
ACCESSION NO.:NP_002277
PROTEIN GI NO.:167614500
GENE ID:3902
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
REFERENCES:1) Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
2) Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
3) Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
4) Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

ANTIBODIES FOR RESEARCH USE ONLY.

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