Datasheet

L1TD1 Antibody
CATALOG NUMBER: 6439

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (76%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:L1TD1 antibody can be used for detection of L1TD1 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
SPECIFICITY:L1TD1 antibody is predicted to not cross-react with other ECAT protein family members
IMMUNOGEN:L1TD1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human L1TD1.

The immunogen is located within amino acids 690 - 740 of L1TD1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:L1TD1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:L1TD1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:L1TD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:L1TD1 Antibody: ECAT11, ECAT11, LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11
ACCESSION NO.:NP_061952
PROTEIN GI NO.:157694505
OFFICIAL SYMBOL:L1TD1
GENE ID:54596

Background

BACKGROUND:L1TD1 Antibody: L1TD1 (ECAT11) is a member of the family of Embryonic Stem Cell Associated Transcripts (ECAT) that play an important role in stem cell biology. ECAT4 (Nanog) is a master regulator for the maintenance of mESC and hESC. ECAT5 was identified to be Embryonic stem cell expressed Ras (ERas), a Ras-like oncogene that is important in regulating mESC proliferation. ECAT9 (GDF3) is an important factor that helps maintain mESC pluripotency by inhibiting bone morphogenetic protein (BMP) signaling. L1TD1 is highly enriched in undifferentiated hESC and L1TD1 is a target gene of Nanog.
REFERENCES: 1) Iwabuchi KA, Yamakawa T, Sato Y, et al. ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency. PLoS One. 2011; 6:e20461.
2) Mitsui K, Tokuzawa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 2003; 113:631-42
3) Takahashi K, Mitsui K, Yamanaka S. Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. Nature 2003; 423:541-5
4) Wong RC, Ibrahim A, Fong H, et al. L1TD1 is a marker for undifferentiated human embryonic stem cells. PLoS One 2011;6:e19355.

For Research Use Only