KREMEN2 Antibody Cat. No.: 7263

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-KREMEN2 antibody (7263) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2.

The immunogen is located within amino acids 390-440 of KREMEN2.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: WB: 1-2 μg/mL; ICC: 5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 49kD

Observed: 49kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar KREMEN2 expression profile in human, mouse and rat cell lines detected by two independent anti-KREMEN2 antibodies that recognize different epitopes, 7263 against C-terminus domain and 64-235 against central domain. KREMEN2 proteins are detected in most of the tested cell lines at different expression levels by the two independent antibodies.

ISOFORMS: Human KREMEN2 has 6 isoforms, including isoform 1 (462aa, 49kD), isoform 2 (424aa, 45kD), isoform 3 (420aa, 44kD), isoform 4 (399aa, 42kD), isoform 5 (423aa, 45kD), and isoform 6 (381aa, 40kD). Mouse KREMEN2 has one isoform (461aa, 49kD) and rat KREMEN2 also has one isoform (468aa, 50kD). 7263 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:KREMEN2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KREMEN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KREMEN2
ALTERNATE NAMES:KREMEN2 Antibody: KRM2, KRM2, Kremen protein 2, Dickkopf receptor 2
ACCESSION NO.:NP_757384
PROTEIN GI NO.:27437008
GENE ID:79412
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KREMEN2 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
REFERENCES:1) Nakamura et al. Biochim. Biophys. Acta. 2001; 1518:63-72.
2) Mao et al. Nature 2002; 417:664-7.
3) Li et al. Fertil. Steril. 2008; 90:1470-9.
4) Schulze et al. PLoS 2010; 5:e10309.

ANTIBODIES FOR RESEARCH USE ONLY.

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