KREMEN2 Antibody Cat. No.: 7263

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: KREMEN2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human KREMEN2 .

The immunogen is located within amino acids 390 - 440 of KREMEN2.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: KREMEN2 Antibody can be used for detection of KREMEN2 by Western blot starting at 1 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 51 kDa

Observed: 54 kDa

psi-iconProperties
PURIFICATION:KREMEN2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KREMEN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KREMEN2
ALTERNATE NAMES:KREMEN2 Antibody: KRM2, KRM2, Kremen protein 2, Dickkopf receptor 2
ACCESSION NO.:NP_757384
PROTEIN GI NO.:27437008
GENE ID:79412
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KREMEN2 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
REFERENCES:1) Nakamura T, Aoki S, Kitajima K, et al. Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein. Biochim. Biophys. Acta. 2001; 1518:63-72.
2) Mao B, Wu W, Davidson G, et al. Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling. Nature 2002; 417:664-7.
3) Li J, Liu WM, Cao YJ, et al. Roles of Dickkopf-1 and its receptor KREMEN1 during embryonic implantation in mice. Fertil. Steril. 2008; 90:1470-9.
4) Schulze J, Seitz S, Saito H, et al. Negative regulation of bone formation by the transmembrane Wnt antagonist Kremen-2. PLoS 2010; 5:e10309.

ANTIBODIES FOR RESEARCH USE ONLY.

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