KREMEN1 Antibody Cat. No.: 7261

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Rabbit polyclonal KREMEN1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human KREMEN1.

The immunogen is located within the last 50 amino acids of KREMEN1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KREMEN1 antibody can be used for detection of KREMEN1 by Western blot at 0.125 - 0.25 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Three isoforms of KREMEN1 exists as a result of alternative splicing event.
POSITIVE CONTROL:1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT: 54 kDa

psi-iconProperties
PURIFICATION:KREMEN1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KREMEN1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KREMEN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KREMEN1
ALTERNATE NAMES:KREMEN1 Antibody: KRM1, KREMEN, KRM1, Kremen protein 1, Dickkopf receptor
ACCESSION NO.:NP_114434
PROTEIN GI NO.:24041012
GENE ID:83999
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KREMEN1 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) coreceptor. KREMEN1 is a receptor for the Dickkopf protein which blocks Wnt/beta catenin signaling. It is necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes.
REFERENCES:1) Nakamura T, Aoki S, Kitajima K, et al. Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein. Biochim. Biophys. Acta. 2001; 1518:63-72.
2) Mao B, Wu W, Davidson G, et al. Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling. Nature 2002; 417:664-7.
3) Li J, Liu WM, Cao YJ, et al. Roles of Dickkopf-1 and its receptor Kremen1 during embryonic implantation in mice. Fertil. Steril. 2008; 90:1470-9.
4) Wang K, Zhang Y, Li X, et al. Characterization of the Kremen-binding site on Dkk1 and elucidation of the role of Kremen in Dkk-mediated Wnt antagonism. J. Biol. Chem. 2008; 283:23371-5.

ANTIBODIES FOR RESEARCH USE ONLY.

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