Datasheet

KPNA6 Antibody
CATALOG NUMBER: 5991

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (92%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:KPNA6 antibody can be used for detection of KPNA6 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
IMMUNOGEN:KPNA6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human KPNA6.

The immunogen is located within amino acids 70 - 120 of KPNA6.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KPNA6 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KPNA6 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KPNA6 Antibody: IPOA7, KPNA7, IPOA7, Importin subunit alpha-7, Karyopherin subunit alpha-6
ACCESSION NO.:CAH71948
PROTEIN GI NO.:55664992
OFFICIAL SYMBOL:KPNA6
GENE ID:23633

Background

BACKGROUND:KPNA6 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.
REFERENCES: 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
3) Kohler MC, Fiebeler M, Hartwig S, et al. Evidence for distinct substrate specificity of importin alpha family members in nuclear protein import. Mol. Cell. Biol.1999; 19:7782-91.
4) Quensal C, Friedrich B, Sommer T, et al. In vivo analysis of importin a proteins reveals cellular proliferation inhibition and substrate specificity. Mol. Cell. Biol.2004; 24:10246-55.

For Research Use Only