Datasheet

KPNA5 Antibody
CATALOG NUMBER: 6655

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (94%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 17-207 - EL4 Cell Slide
SPECIFICITY:KPNA5 antibody is predicted to not cross-react with other Importin alpha family members.
IMMUNOGEN:KPNA5 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human KPNA5.

The immunogen is located within amino acids 50 - 100 of KPNA5.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KPNA5 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KPNA5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KPNA5 Antibody: SRP6, IPOA6, Importin subunit alpha-6, Karyopherin subunit alpha-5
ACCESSION NO.:CAH71948
PROTEIN GI NO.:55664992
OFFICIAL SYMBOL:KPNA5
GENE ID:3841

Background

BACKGROUND:KPNA5 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein imort into the nucleus.
REFERENCES: 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene. Expr. 1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem. 2003; 278: 51937-49
3) Kohler MC, Fiebeler M, Hartwig S, et al. Evidence for distinct substrate specificity of importin alpha family members in nuclear protein import. Mol. Cell. Biol. 1999; 19:7782-91.

For Research Use Only