Datasheet

KPNA4 Antibody
CATALOG NUMBER: 5987

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
IMMUNOGEN:KPNA4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA4.

The immunogen is located within amino acids 30 - 80 of KPNA4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KPNA4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KPNA4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KPNA4 Antibody: QIP1, SRP3, IPOA3, QIP1, Importin subunit alpha-3, Importin alpha Q1, Qip1
ACCESSION NO.:EAW78632
PROTEIN GI NO.:119599038
OFFICIAL SYMBOL:KPNA4
GENE ID:3840

Background

BACKGROUND:KPNA4 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. Molecules containing the classical nuclear localization signal (NLS) are transported into the nucleus by alpha/beta heterodimers. KPNA3 has been shown to be important in the TNF-α-induced nuclear import of NF-κB. It is also involved in the stress-mediated nuclear stabilization of p53 and the nuclear import and replication of HIV-1 in both dividing and non-dividing cells.
REFERENCES: 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
3) Goldfarb DS, Corbett AH, Mason DA, et al. Importin alpha: a multi-purpose nuclear-transport receptor. Trends Cell Biol.2004; 14:505-14.
4) Fagerlund R, Melen K, Cao X, et al. NF-kappaB p52, RelB, and c-Rel are transported into the nucleus via a subset of importin alpha molecules. Cell Signal.2008; 20:1442-51.

For Research Use Only