Datasheet

KPNA3 Antibody
CATALOG NUMBER: 5985

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:KPNA3 antibody can be used for detection of KPNA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
 3) Cat. No. 17-207 - EL4 Cell Slide
IMMUNOGEN:KPNA3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy end of human KPNA3.

The immunogen is located within the last 50 amino acids of KPNA3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KPNA3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KPNA3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KPNA3 Antibody: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma, QIP2, Importin subunit alpha-4, Importin alpha Q2, Qip2
ACCESSION NO.:NP_002258
PROTEIN GI NO.:34485722
OFFICIAL SYMBOL:KPNA3
GENE ID:3839

Background

BACKGROUND:KPNA3 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA3, the alpha subunit 3 of karyopherin, is silimar to human KPNA2, suggesting that KPNA3 may be involved in the nuclear transport system. It is ubiquitously expressed and recent studies have shown that human KPNA3 may be associated with schizophrenia.
REFERENCES: 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
3) Takeda S, Fujiwara T, Shimizu F, et al. Isolation and mapping of karyopherin alpha 3 (KPNA3), a human gene that is highly homologous to genes encoding Xenopus importin, yeast SRP1 and human RCH1. Cytogenet. Cell Genet.1997; 76:87-93.
4) Zhang H, Ju G, Wei J, et al. A combined effect of the KPNA3 and KPNB3 genes on susceptibility to schizophrenia. Neurosci. Lett.2006; 402:173-5.

For Research Use Only