Datasheet

KPNA2 Antibody
CATALOG NUMBER: 5983

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:KPNA2 antibody can be used for detection of KPNA2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1461 - Rat Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
 3) Cat. No. 1224 – Daudi Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 55 kDa

Observed: 58 kDa
IMMUNOGEN:KPNA2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA2.

The immunogen is located within amino acids 40 - 90 of KPNA2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KPNA2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KPNA2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KPNA2 Antibody: QIP2, RCH1, IPOA1, SRP1alpha, SRP1, Importin subunit alpha-1, Karyopherin subunit alpha-2
ACCESSION NO.:NP_002257
PROTEIN GI NO.:4504897
OFFICIAL SYMBOL:KPNA2
GENE ID:3838

Background

BACKGROUND:KPNA2 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA2 is the alpha subunit 2 of karyopherin, which forms a complex with importin subunit beta-1 and functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. It is ubiquitously expressed and contains an IBB/importin beta domain, ten Armadillo repeats that bind "cargo" and three intervening nuclear localization sequences (NLSs). It has recently been reported to play an important role in tumorigenesis and tumor progression.
REFERENCES: 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
3) Tseng SF, Chang CY, Wu KJ, et al. Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1. J. Biol. Chem.2005; 280:39594-600.
4) Sakai M, Sohda M, Miyazaki T, et al. Significance of karyopherin-{alpha} 2 (KPNA2) expression in esophageal squamous cell carcinoma. Anticancer Res.2010; 30:851-6.

For Research Use Only