KPNA2 Antibody Cat. No.: 5983

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: KPNA2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA2.

The immunogen is located within amino acids 40 - 90 of KPNA2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KPNA2 antibody can be used for detection of KPNA2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1461 - Rat Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
 3) Cat. No. 1224 – Daudi Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 55 kDa

Observed: 58 kDa

psi-iconProperties
PURIFICATION:KPNA2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KPNA2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:KPNA2
ALTERNATE NAMES:KPNA2 Antibody: QIP2, RCH1, IPOA1, SRP1alpha, SRP1, Importin subunit alpha-1, Karyopherin subunit alpha-2
ACCESSION NO.:NP_002257
PROTEIN GI NO.:4504897
GENE ID:3838
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KPNA2 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA2 is the alpha subunit 2 of karyopherin, which forms a complex with importin subunit beta-1 and functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. It is ubiquitously expressed and contains an IBB/importin beta domain, ten Armadillo repeats that bind "cargo" and three intervening nuclear localization sequences (NLSs). It has recently been reported to play an important role in tumorigenesis and tumor progression.
REFERENCES:1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
3) Tseng SF, Chang CY, Wu KJ, et al. Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1. J. Biol. Chem.2005; 280:39594-600.
4) Sakai M, Sohda M, Miyazaki T, et al. Significance of karyopherin-{alpha} 2 (KPNA2) expression in esophageal squamous cell carcinoma. Anticancer Res.2010; 30:851-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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