Datasheet

KPNA1 Antibody
CATALOG NUMBER: 5981

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:KPNA1 antibody can be used for detection of KPNA1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
 3) Cat. No. 17-004 - K-562 Cell Slide
IMMUNOGEN:KPNA1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA1.

The immunogen is located within amino acids 30 - 80 of KPNA1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KPNA1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KPNA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KPNA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KPNA1 Antibody: RCH2, SRP1, IPOA5, NPI-1, RCH2, Importin subunit alpha-5, Karyopherin subunit alpha-1
ACCESSION NO.:NP_002255
PROTEIN GI NO.:222144293
OFFICIAL SYMBOL:KPNA1
GENE ID:3836

Background

BACKGROUND:KPNA1 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA1, also known as importin alpha 5, is the alpha subunit of karyopherin, which forms a complex with importin subunit beta-1 and functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. It is ubiquitously expressed and polyubiquitinated in the presence of RAG1. KPNA1 interacts with various virus nucleoproteins, including those of Ebola and influenza.
REFERENCES: 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
3) Simkus C, Makiya M and Jones JM. Karyopherin alpha 1 is a putative substrate of the RAG1 ubiquitin ligase. Mol. Immunol.2009; 46:1319-25.
4) Reid SP, Valmas C, and Martinez O. Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1. J. Virol.2007; 81:13469-77.

For Research Use Only